TLC Visualization reagents

THIN LAYER CHROMATOGRAPHY VISUALIZATION REAGENTS

Ninhydrin -
Part A: Ninhydrin 100 mg x 4 vials
Part B: 2,4,6-Collidine in Methanol, 200 mL
Precaution: In vitro diagnostic reagents - For Laboratory Use Only. Dust Hazardous: Avoid contact with skin, eyes, or clothing.
Stability: See expiration date on label. Store unreconstituted in dark at 15-30⁰C. Stable for three weeks after reconstitution when stored in cool, dark location.
Reconstitution: 2 mg of Ninhydrin is required for each 1 mL of diluent used.

Diphenylcarbazone -
Part A: Diphenylcarbazone, 47 mg
Part B: Acetone:Water (1:1), 500 mL
Precaution: In vitro diagnostic reagent - For Laboratory Use Only. May be irritating to skin and mucous membranes. Light sensitive.
Stability: See expiration date on label. Store unreconstituted in dark at 15-30⁰C. Stable for at least one working day after reconstitution. The color of the solution is a good indicator of whether it still may be used. It is usable until the color fades significantly from its original intensity of orange.
Reconstitution: Approximately 0.2 mg of diphenylcarbazone is required for each 2 mL of diluent used.
Mercuric Sulfate Solution - 0.25% Mercuric Oxide in 10% Sulfuric Acid, 16 oz.
Precaution: ln vitro diagnostic reagent. For Laboratory Use Only. Poison. Do not pipet by mouth. If swallowed call a physician immediately. Irritating to skin and mucous membranes. Corrosive - causes burns to skin and eyes. Get immediate medical attention for eyes.
Stability: See expiration date on label. Store tightly closed at 15-30⁰C.
lodoplatinate -
Solution A: 0.79% Chloroplatinic Acid in H20, 240 mL
Solution B: 19.35% KI in H20, 240 mL
Precaution: in vitro diagnostic reagent. For laboratory use only. May be irritating to skin and mucous membranes.
Stability: See expiration date on label. Stable for two weeks after mixing when refrigerated.
Preparation: Mix equal parts of A and B
Dragendorf Solution -
Part A: 0.95% BIsmuth Subnitrate in 30.6% Acetic Acid, 240 mL
Part B: 11.21% KI in H20, 240 mL
Precaution: In vitro diagnostic reagent. For laboratory use only. Corrosive reagent causes burns to skin and eyes. Get immediate medical attention for eyes.
Stability: See expiration date on label. Store tightly closed at room temperature. Light sensitive, store In dark. Stable for two weeks after mixing when kept refrigerated and protected from light.
Preparation: Mix equal parts of A and B.
OPTIONAL SPRAY REAGENTS (Available as a custom reagent)
Ammoniacal Silver Nitrate - 50% AgNO3:5N NH4OH (1:1) Precaution: Irritating to skin and mucous membranes. Poison - may be fatal if swallowed. Get immediate medical attention.
Preparation: 50% AgNO3 is mixed with equal parts of 5N NH4OH immediately prior to use. If cloudy add NH4OH drop by drop until solution clears.
Sodium Borohydride - 10% in 1N NaOH
Precaution: Caustic reagent - causes burns to skin and eyes. Get immediate medical attention for eyes.
Preparation: Prepare only enough reagent for one day's work.
Sodium Nitrite - 2% in 1.5N HCI
Precaution: Corrosive - causes burns to skin and eyes. Get immediate medical attention for eyes.
Stability: Slowly oxidized to nitrate when exposed to air.
N-(1-Napthyl) Ethylenediamine - 2% Aqueous Solution
Precaution: May be irritating to eyes and mucous membranes. Poisoning may occur by ingestion.
Stability: Stable for at least two months when stored in dark, cool location.
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Ninhydrin - Part A: Ninhydrin 100 mg x 4 vials Part B: 2,4,6-Collidine in Methanol, 200 mL
Precaution: In vitro diagnostic reagents - For Laboratory Use Only. Dust Hazardous: Avoid contact with skin, eyes, or clothing.
Stability: See expiration date on label. Store unreconstituted in dark at 15-30⁰C. Stable for three weeks after reconstitution when stored in cool, dark location.
Reconstitution: 2 mg of Ninhydrin is required for each 1 mL of diluent used.
Diphenylcarbazone - Part A: Diphenylcarbazone, 47 mg Part B: Acetone:Water (1:1), 500 mL
Precaution: In vitro diagnostic reagent - For Laboratory Use Only. May be irritating to skin and mucous membranes. Light sensitive.
Stability: See expiration date on label. Store unreconstituted in dark at 15-30⁰C. Stable for at least one working day after reconstitution. The color of the solution is a good indicator of whether it still may be used. It is usable until the color fades significantly from its original intensity of orange.
Reconstitution: Approximately 0.2 mg of diphenylcarbazone is required for each 2 mL of diluent used.
Mercuric Sulfate Solution - 0.25% Mercuric Oxide in 10% Sulfuric Acid, 16 oz.
Precaution: ln vitro diagnostic reagent. For Laboratory Use Only. Poison. Do not pipet by mouth. If swallowed call a physician immediately. Irritating to skin and mucous membranes. Corrosive - causes burns to skin and eyes. Get immediate medical attention for eyes.
Stability: See expiration date on label. Store tightly closed at 15-30⁰C.
lodoplatinate - Solution A: 0.79% Chloroplatinic Acid in H20, 240 mL Solution B: 19.35% KI in H20, 240 mL
Precaution: in vitro diagnostic reagent. For laboratory use only. May be irritating to skin and mucous membranes.
Stability: See expiration date on label. Stable for two weeks after mixing when refrigerated.
Preparation: Mix equal parts of A and B
Dragendorf Solution - Part A: 0.95% BIsmuth Subnitrate in 30.6% Acetic Acid, 240 mL Part B: 11.21% KI in H20, 240 mL
Precaution: In vitro diagnostic reagent. For laboratory use only. Corrosive reagent causes burns to skin and eyes. Get immediate medical attention for eyes.
Stability: See expiration date on label. Store tightly closed at room temperature. Light sensitive, store In dark. Stable for two weeks after mixing when kept refrigerated and protected from light.
Preparation: Mix equal parts of A and B.
OPTIONAL SPRAY REAGENTS (Available as a custom reagent)
Ammoniacal Silver Nitrate - 50% AgNO3:5N NH4OH (1:1) Precaution: Irritating to skin and mucous membranes. Poison - may be fatal if swallowed. Get immediate medical attention.
Preparation: 50% AgNO3 is mixed with equal parts of 5N NH4OH immediately prior to use. If cloudy add NH4OH drop by drop until solution clears.
Sodium Borohydride - 10% in 1N NaOH
Precaution: Caustic reagent - causes burns to skin and eyes. Get immediate medical attention for eyes.
Preparation: Prepare only enough reagent for one day's work.
Sodium Nitrite - 2% in 1.5N HCI
Precaution: Corrosive - causes burns to skin and eyes. Get immediate medical attention for eyes.
Stability: Slowly oxidized to nitrate when exposed to air.
N-(1-Napthyl) Ethylenediamine - 2% Aqueous Solution
Precaution: May be irritating to eyes and mucous membranes. Poisoning may occur by ingestion.
Stability: Stable for at least two months when stored in dark, cool location.
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