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MULTI-PREP® GRAVITY SERIES GVSA COLUMN METHODS FOR THE ANALYSIS OF METHYLMALONIC ACID AND HOMOCYSTEINE IN SERUM USING GC-MS |
| There is increased interest in measuring Methylmalonic Acid and
Homocysteine in serum because recent studies indicate that abnormal levels
of these compounds are used in distinguishing between Cobalamin (Vitamin
B12) and Folate deficiencies. Serum levels of Total Homocysteine and Methylmalonic
Acid are elevated in most patients with Cobalamin deficiency, whereas
the Total Homocysteine level alone is elevated in most patients with Folate
deficiency.
In addition, the measurement of Total Homocysteine levels in serum
is becoming more prevalent because of strong evidence presented in recent
studies showing a high correlation between elevated levels of this compound
and a variety of vascular diseases including strokes, heart attacks, and
peripheral vascular disease.
Extraction methods using the Multi-Prep
GVSA-200 column for the quantitative analysis of Methylmalonic Acid and
the Multi-Prep GVSA-100 column for the quantitative analysis of Homocysteine
in serum have been developed. These methods involve sample preparation on
the Multi-Prep GVSA column, a strong anion exchange gravity flow column,
and measurement by GC-MS using MMA-D3 and Homocystine-D8 as internal standards.
Homocysteine is protected from oxidation with the use of a proprietary stabilizer.
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