The extraction methods listed below are suitable for GC/MS and LC/MS. For LC/MS it is best to substitute ammonia for TEA and either dry down the sample prior to taking it up in mobile phase, or, modify the elution solvent, (e.g. basic methanol or water methanol mixtures), to insure that it is compatible with the mobile phase. We can also provide you with a simplifed two step procedure for basic compounds that does not involve cation exchange, which, because of the small sample volumes typically used in LC/MS, produces excellent results. Please contact us for guidance on this subject.

Prevention of cross contamination and carryover has always been important but as methods become more sensitive this issue has come to the forefront of laboratory inspections and evaluations. Please refer to the "hardware Selection Overview" to see how our hardware design and methods are designed to help you eliminate this worrysome problem.

The water wash following methanol column activation is optional and applies to columns activated but not used for more than 20 minutes such as might happen when used on a robotics platform Such delays may lead to methanol evaporation which can cause a loss in column efficiency.

We also suggest that you become familiar with our troubleshooting guide while setting up any of the following extraction procedures.

Cocaine and Metabolites
pdf format

Revised 5/11/11 to substitute Acetonitrile for Ethyl Acetate from the derivatization step to help avoid possible conversion of Opiates to their Acetyl form - e.g. 6-MAM

Extended Opiates
pdf format

Revised 7/11 to add acid hydrolysis to the procedure.

including: (but not limited to):

Vitamin D2,D3
method - pdf format
Reference publication- Agilent Technologies :